Logo Logo
  • Home
  • Publications
  • Meet Our Team
  • Contact

More Info

  • Email [email protected]
  • Phone Office: (434) 924-8195 Computational lab: (434) 982-6267 Wet lab: (434) 924-8640
  • Location 415 Lane Road, Room 2041 Charlottesville, VA 22903

Related Links

  • PubMed
  • Undergraduate Opportunities
  • Graduate Opportunities
  • UVA Engineering

Connect With Us

Untargeted Metabolomics Reveals Species-Specific Metabolite Production and Shared Nutrient Consumption by Pseudomonas aeruginosa and Staphylococcus aureus

  • Home
  • Blog Details
June 22 2021
  • Published Works

mSystems. 2021 Jun 29;6(3):e0048021. doi: 10.1128/mSystems.00480-21. Epub 2021 Jun 22.

ABSTRACT

While bacterial metabolism is known to impact antibiotic efficacy and virulence, the metabolic capacities of individual microbes in cystic fibrosis lung infections are difficult to disentangle from sputum samples. Here, we show that untargeted metabolomic profiling of supernatants of multiple strains of Pseudomonas aeruginosa and Staphylococcus aureus grown in monoculture in synthetic cystic fibrosis media (SCFM) reveals distinct species-specific metabolic signatures despite intraspecies metabolic variability. We identify a set of 15 metabolites that were significantly consumed by both P. aeruginosa and S. aureus, suggesting that nutrient competition has the potential to impact community dynamics even in the absence of other pathogen-pathogen interactions. Finally, metabolites that were uniquely produced by one species or the other were identified. Specifically, the virulence factor precursor anthranilic acid, as well as the quinoline 2,4-quinolinediol (DHQ), were robustly produced across all tested strains of P. aeruginosa. Through the direct comparison of the extracellular metabolism of P. aeruginosa and S. aureus in a physiologically relevant environment, this work provides insight toward the potential for metabolic interactions in vivo and supports the development of species-specific diagnostic markers of infection. IMPORTANCE Interactions between P. aeruginosa and S. aureus can impact pathogenicity and antimicrobial efficacy. In this study, we aim to better understand the potential for metabolic interactions between P. aeruginosa and S. aureus in an environment resembling the cystic fibrosis lung. We find that S. aureus and P. aeruginosa consume many of the same nutrients, suggesting that metabolic competition may play an important role in community dynamics during coinfection. We further identify metabolites uniquely produced by either organism with the potential to be developed into species-specific biomarkers of infection in the cystic fibrosis lung.

PMID:34156287 | PMC:PMC8269234 | DOI:10.1128/mSystems.00480-21

Previous Post Next Post

Recent Posts

  • Fecal sphingolipids predict parenteral nutrition associated cholestasis in the neonatal intensive care unit
  • Comparative analyses of parasites with a comprehensive database of genome-scale metabolic models
  • Comparative analyses of parasites with a comprehensive database of geno-scale metabolic models
  • Quantifying cumulative phenotypic and genomic evidence for procedural generation of metabolic network reconstructions
  • Computational approaches to understanding Clostridioides difficile metabolism and virulence
Logo

Computational Systems Biology Laboratory; The research group of Dr. Jason Papin in the Department of Biomedical Engineering at the University of Virginia. Dedicated to discovering revolutionary advancements.

Related Links

  • PubMed
  • Undergraduate Opportunities
  • Graduate Opportunities
  • UVA Engineering

Contact Info

The research group of Dr. Jason Papin in the Department of Biomedical Engineering at the University of Virginia

  • Email: [email protected]
  • Phone (434) 924-8195

© Copyright 2021 Papin Lab. Designed by Sabres Media LLC

  • Home
  • Publications
  • Meet Our Team
  • Contact